Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7)

Synonyms: CHAK, CHAK1, LTRPC7, FLJ20117, FLJ25718, TRP-PLIK, Ltpr7, Ltrpc7, LTrpC-7, TRPPLIK, 2310022G15Rik, 4833414K03Rik, 5033407O22Rik, cb495, TRPM7, Chak, Trp-plik, chak, chak1, ltrpc7, trp-plik

Clonality: Monoclonal (S74-25)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN361775

Additional Information

Characteristics: Detects ~220kDa. No cross-reactivity against TrpM6

Alternative name: TRPM7

Gene ID: 58800

Swiss-Prot: Q923J1

Immunogen: Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7, accession number Q923J1

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S74-25

Description: TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants .
Synonyms: CHAK, CHAK1, LTRPC7, TRP PLIK, Channel kinase 1

Specificity: ~220kDa. No cross reactivity with TrpM6. Species cross-reactivity: Human, Mouse, Rat.

Sensitivity: 1 µg/mL of SMC-316 was sufficient for detection of TrpM7 in 10µg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

TrpM7 (S74-25), Human cell line mix

TrpM7 (S74-25), Human hippocampus

Publications

Publications: Numata, Okada: "Proton conductivity through the human TRPM7 channel and its molecular determinants." in: The Journal of biological chemistry, Vol. 283, Issue 22, pp. 15097-103, 2008 (PubMed).

Brauchi, Krapivinsky, Krapivinsky et al.: "TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 24, pp. 8304-8, 2008 (PubMed).