TRPM7 antibody (AA 1817-1863)

Catalog No. ABIN361775

The Mouse Monoclonal anti-TRPM7 antibody is suitable to detect TRPM7 in samples from Mouse. It has been validated for WB, IHC, ICC, IF and AA.

Quick Overview for TRPM7 antibody (AA 1817-1863) (ABIN361775)

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRPM7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Clone: S74

Product Details anti-TRPM7 Antibody

Binding Specificity: AA 1817-1863

Specificity: Detects ~220 kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN361775 was sufficient for detection of TrpM7 in 10 μg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for TRPM7

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Alternative Name: TRPM7

Background: TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (1, 2).

Gene ID: 58800

NCBI Accession: NP_001157797

UniProt: Q923J1