HLA-DR-gamma (CD74) antibody

Antigen: HLA-DR-gamma (CD74)

Synonyms: DHLAG, HLADG, Ia-GAMMA, Ii, CLIP

Clonality: Monoclonal (PIN-1)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Flow Cytometry (FACS)

Catalog no.: ABIN361788

Additional Information

Alternative name: Cd74

Immunogen: Human CD74 invariant chain sythetic peptide

Format: Ig-fraction

Isotype: IgG  (Matching secondary antibodies)

Clone: PIN-1

Description: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

Specificity: Detects ~33-35 kDa protein doublet, corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS-PAGE immunoblots. Species cross-reactivity: Human.

Application Details

Application Notes: 1 ug/mL was sufficient for detection using colorimetric western blot analysis

Concentration: 1.0mg/mL

Buffer: Mouse immunoglobulin in PBS, pH 7.2, 0.09% azide and 50% glycerol

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

CD-74 (PIN 1.1) N87 lysates mixed with Macrophage inhibitory factor

Publications

Publications: Denzin, Robbins, Carboy-Newcomb et al.: "Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells." in: Immunity, Vol. 1, Issue 7, pp. 595-606, 1995 (PubMed).

Denzin, Hammond, Cresswell: "HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2153-65, 1997 (PubMed).

Burton, Ely, Reddy et al.: "CD74 is expressed by multiple myeloma and is a promising target for therapy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 19, pp. 6606-11, 2004 (PubMed).

Barrera, Beswick, Sierra et al.: "Polarized expression of CD74 by gastric epithelial cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 53, Issue 12, pp. 1481-9, 2005 (PubMed).

Becker-Herman, Arie, Medvedovsky et al.: "CD74 is a member of the regulated intramembrane proteolysis-processed protein family." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5061-9, 2005 (PubMed).

Starlets, Gore, Binsky et al.: "Cell-surface CD74 initiates a signaling cascade leading to cell proliferation and survival." in: Blood, Vol. 107, Issue 12, pp. 4807-16, 2006 (PubMed).