anti-FKBP5 antibody (FK506 Binding Protein 5)

Details for Product anti-FKBP5 Antibody No. ABIN361794
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Antigen
Synonyms FKBP5, FKBP-5, AIG6, FKBP51, FKBP54, P54, PPIase, Ptg-10, D17Ertd592e, Dit1, si:zc263a23.8, wu:fc31g11, wu:fl87b03, zgc:64082
Reactivity
Dog (Canine), Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus)
(93), (47), (40), (23), (22), (21), (2), (2), (1), (1), (1), (1), (1)
Host
Mouse
(53), (45), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
This FKBP5 antibody is un-conjugated
(7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(83), (48), (26), (23), (14), (12), (10), (4), (3), (3), (1)
Pubmed 5 references available
Quantity 25 μg
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Immunogen Synthetic peptide corresponding to the residues of human FKBP51
Clone Hi51B
Specificity Detects ~51 kDa.
Sensitivity A 1:2000 dilution was sufficient for detection of FKBP51 in ~50 µg total protein using WB anaylsis.
Purification Protein G Purified
Alternative Name FKBP51 (FKBP5 Antibody Abstract)
Background HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
Cellular Localization: Cytoplasm | Nucleus
Gene ID 2289
NCBI Accession NP_001139247
UniProt Q13451
Research Area Signaling, Heat Shock Proteins
Application Notes Recommended Dilution: WB (1:2000)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-FK506 Binding Protein 5 (FKBP5) antibody FKBP51 HS Hela 10ug 1 in 1000 Western Blotting copy.
Product cited in: Mata-Greenwood, Jackson, Pearce et al.: "Endothelial glucocorticoid receptor promoter methylation according to dexamethasone sensitivity." in: Journal of molecular endocrinology, 2015 (PubMed).

Background publications Denny, Prapapanich, Smith et al.: "Structure-function analysis of squirrel monkey FK506-binding protein 51, a potent inhibitor of glucocorticoid receptor activity." in: Endocrinology, Vol. 146, Issue 7, pp. 3194-201, 2005 (PubMed).

Wu, Li, Liu et al.: "3D structure of human FK506-binding protein 52: implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin heterocomplex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 22, pp. 8348-53, 2004 (PubMed).

Hubler, Denny, Valentine et al.: "The FK506-binding immunophilin FKBP51 is transcriptionally regulated by progestin and attenuates progestin responsiveness." in: Endocrinology, Vol. 144, Issue 6, pp. 2380-7, 2003 (PubMed).

Davies, Ning, Sánchez: "A new first step in activation of steroid receptors: hormone-induced switching of FKBP51 and FKBP52 immunophilins." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4597-600, 2002 (PubMed).

Validation Images
Catalog No. ABIN361794
217.80 $
Plus shipping costs $45.00
Quantity
Price
25 μg
217.80 $

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