Rab5 antibody

Antigen: Rab5

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Monkey, Cow (Bovine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361845

Additional Information

Immunogen: Human Rab5 synthetic peptide conjugated to KLH, identical to dog Rab5 sequence over the residues

Format: Affinity-purified

Description: Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.

Specificity: The antibody recognizes 26 kDa Rab5 of human, mouse, monkey, bovine and rat origins. Species cross-reactivity: Human, Mouse, Monkey, Bovine and Rat. Reactivity to other species has not been tested.

Application Details

Application Notes: Recommended dilution for WB 1:1000

Buffer: Protein A Purified. In 1x PBS, 50% glycerol and 0.09% sodium azide.

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: Neurology, Organelles, Signaling

Restrictions: For Research Use only

Images

Rab5, mouse backskin

Publications

Publications: Soennichsen, De Renzis, Nielsen et al.: "Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11." in: The Journal of cell biology, Vol. 149, Issue 4, pp. 901-14, 2000 (PubMed).

Takai, Sasaki, Matozaki: "Small GTP-binding proteins." in: Physiological reviews, Vol. 81, Issue 1, pp. 153-208, 2001 (PubMed).

Woodman: "Biogenesis of the sorting endosome: the role of Rab5." in: Traffic (Copenhagen, Denmark), Vol. 1, Issue 9, pp. 695-701, 2001 (PubMed).

Zerial, McBride: "Rab proteins as membrane organizers." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 2, pp. 107-17, 2001 (PubMed).

Stenmark, Olkkonen: "The Rab GTPase family." in: Genome biology, Vol. 2, Issue 5, pp. REVIEWS3007, 2001 (PubMed).

Ali, Wasmeier, Lamoreux et al.: "Multiple regions contribute to membrane targeting of Rab GTPases." in: Journal of cell science, Vol. 117, Issue Pt 26, pp. 6401-12, 2004 (PubMed).