Add to Basket
|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Human, Monkey, Mouse (Murine), Rat (Rattus), Cow (Bovine), Rabbit, Pig (Porcine), Dog (Canine), Hamster, Chicken, Sheep (Ovine), Guinea Pig
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
|7 references available|
|Price||152.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Immunogen||A 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH.|
The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway. The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2alpha translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death. Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP. p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs. As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis and has also been implicated in cancer, CNS and diabetes.
Synonyms: CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, MAPK14, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A
|Characteristics||Accession Number: NP_001306.1|
|Specificity||Detects a ~43kDa protein corresponding to the molecular mass of p38 on SDS PAGE immunoblots.|
|Application Notes||1:5000 (ECL) (WB), 1:250 (IP)|
|Storage||Store at -20° C. Shipping Temperature: Blue Ice or 4° C|
|Storage Shipping Temp Max||Blue Ice or 4 °C|
|Research Area||Cell Signaling, Immunology, Innate Immunity, Tyrosine Kinases|
|Restrictions||For Research Use only|
Han, Lee, Bibbs et al.: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).
Pearson, Robinson, Beers Gibson et al.: "Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions." in: Endocrine reviews, Vol. 22, Issue 2, pp. 153-83, 2001 (PubMed).
Deng, Ren, Yang et al.: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003 (PubMed).
Van Laethem, Van Kelst, Lippens et al.: "Activation of p38 MAPK is required for Bax translocation to mitochondria, cytochrome c release and apoptosis induced by UVB irradiation in human keratinocytes." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 15, pp. 1946-8, 2004 (PubMed).
Salojin, Owusu, Millerchip et al.: "Essential role of MAPK phosphatase-1 in the negative control of innate immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 3, pp. 1899-907, 2006 (PubMed).
Medicherla, Protter, Ma et al.: "Preventive and therapeutic potential of p38 alpha-selective mitogen-activated protein kinase inhibitor in nonobese diabetic mice with type 1 diabetes." in: The Journal of pharmacology and experimental therapeutics, Vol. 318, Issue 1, pp. 99-107, 2006 (PubMed).
Fan, Chen, Qiao et al.: "Opposing effects of ERK and p38 MAP kinases on HeLa cell apoptosis induced by dipyrithione." in: Molecules and cells, Vol. 23, Issue 1, pp. 30-8, 2007 (PubMed).
|Reactivities||Human (5), Rat (Rattus) (4), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Guinea Pig (1), Hamster (1), Monkey (1), Mouse (Murine) (1), Pig (Porcine) (1), Rabbit (1), Sheep (Ovine) (1)|
|Applications||Western Blotting (WB) (5), Immunohistochemistry (IHC) (4), Cellular Assay (CA) (1), Dot Blot (Dot) (1), ELISA (1), Enzyme Immunoassay (EIA) (1), Immunoassay (IA) (1), Immunofluorescence (IF) (1), Immunoprecipitation (IP) (1), Immunostaining (ISt) (1)|