LIMK2 antibody (pThr505)

Catalog No. ABIN362109

The Rabbit Polyclonal anti-LIMK2 antibody (ABIN362109) specifically detects LIMK2 in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LIMK2 antibody (pThr505) (ABIN362109)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-LIMK2 Antibody

Binding Specificity: pThr505

Specificity: The antibody detects endogenous level of LIMK2 only when phosphorylated at threonine 505.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr505 (R-Y-T (p) -V-V) derived from Human LIMK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIMK2

Background: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

Molecular Weight: 70 kDa

NCBI Accession: NP_001026

UniProt: P53671