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ZAP70 antibody (pTyr493)

The Rabbit Polyclonal anti-ZAP70 antibody is suitable to detect ZAP70 in samples from Human, Mouse and Rat. It has been validated for IHC.

Catalog No. ABIN362165

$240.90

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Quick Overview for ZAP70 antibody (pTyr493) (ABIN362165)

Target

See all ZAP70 Antibodies

ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This ZAP70 antibody is un-conjugated

Application

Immunohistochemistry (IHC)

Discover our top product ZAP70 Primary Antibody

Binding Specificity
46

42

34

28

21

20

16

10

10

9

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7

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2

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2

2

2

2

2

1

1

1

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1

1

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1

1
pTyr493

Specificity

The antibody detects endogenous level of Zap-70 only when phosphorylated at tyrosine 493.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen

Peptide sequence around phosphorylation site of pTyr493 (S-Y-Y (p) -T-A) derived from Human Zap-70. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype

IgG
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody
ZAP70 Reactivity: Human WB, ICC, ICFC Host: Mouse Monoclonal ZAP-03 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody
ZAP70 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibody
ZAP70 Reactivity: Human IHC, ELISA, FACS, IF Host: Mouse Monoclonal ZAP70-2035 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibody
ZAP70 Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 1F3G9 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 337-600) antibody
ZAP70 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody
ZAP70 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-254) antibody
ZAP70 Reactivity: Human IHC, FACS, IF Host: Mouse Monoclonal 2F3-2 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibody
ZAP70 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 6D9C2 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibody
ZAP70 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal ZAP70-2046 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody
ZAP70 Reactivity: Human WB, IF Host: Rabbit Monoclonal unconjugated

Application Notes

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target

ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name

Zap-70

Background

Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

Molecular Weight

70 kDa

UniProt

P43403

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway