ASK1 antibody (pSer83)

Catalog No. ABIN362193

Cited in 2+ publications. The Rabbit Polyclonal anti-ASK1 antibody (ABIN362193) specifically detects ASK1 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for ASK1 antibody (pSer83) (ABIN362193)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ASK1 Antibody

Binding Specificity: pSer83

Specificity: The antibody detects endogenous level of ASK1 only when phosphorylated at serine 83.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer83 (G-S-S (p) -V-G) derived from Human ASK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-ASK1 antibody (ABIN362193)

Saha, Jiang, Yang, Zhu, Wang, Schimmer, Qiu, Chang: "Targeting p53 via JNK pathway: a novel role of RITA for apoptotic signaling in multiple myeloma." in: PLoS ONE, Vol. 7, Issue 1, pp. e30215, (2012) (PubMed).

Yang, Yu, Guan, Gu, Cao, Wang, Zheng, Xu, Shen, Liu: "ASK1-JNK signaling cascade mediates Ad-ST13-induced apoptosis in colorectal HCT116 cells." in: Journal of cellular biochemistry, Vol. 110, Issue 3, pp. 581-8, (2010) (PubMed).

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background: Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively.

Molecular Weight: 155 kDa

NCBI Accession: NP_005914

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response