DARPP32 antibody (pThr75)

Catalog No. ABIN362205

The Rabbit Polyclonal anti-DARPP32 antibody (ABIN362205) specifically detects DARPP32 in IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DARPP32 antibody (pThr75) (ABIN362205)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-DARPP32 Antibody

Binding Specificity: pThr75

Specificity: The antibody detects endogenous level of DARPP-32 only when phosphorylated at threonine 75.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr75 (A-Y-T (p) -P-P) derived from Human DARPP-32. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: DARPP-32

Background: Inhibitor of protein-phosphatase 1.

Molecular Weight: 32 kDa

NCBI Accession: NP_115568

UniProt: Q9UD71