HDAC4 antibody (pSer632)

Catalog No. ABIN362216

Product Details anti-HDAC4 Antibody

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Binding Specificity: pSer632

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC4 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous level of HDAC4 only when phosphorylated at serine 632.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer632 (A-Q-S (p) -S-P) derived from Human HDAC4. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000  

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-HDAC4 antibody (ABIN362216)

Lim, Luo, Koh, Yang, bin Abdul Kadir, Tan, Ye, Wang, Melamed et al.: "Distinct mechanisms involving diverse histone deacetylases repress expression of the two gonadotropin beta-subunit genes in immature gonadotropes, and their actions are overcome by ..." in: Molecular and cellular biology, Vol. 27, Issue 11, pp. 4105-20, (2007) (PubMed).

Target Details for HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: HDAC4 (HDAC4 Products)

Synonyms: CG1770 antibody, DHDAC4 antibody, Dmel\\CG1770 antibody, GC1770 antibody, HDAC antibody, HDAC4a antibody, dHDAC4 antibody, dmHDA405 antibody, hdac4 antibody, dana_GLEANR_19966 antibody, DanaGF18710 antibody, GF18710 antibody, HDAC4 antibody, AHO3 antibody, BDMR antibody, HA6116 antibody, HD4 antibody, HDAC-4 antibody, HDAC-A antibody, HDACA antibody, 4932408F19Rik antibody, AI047285 antibody, wu:fa96d08 antibody, wu:fc56f08 antibody, zgc:152701 antibody, histone deacetylase 4 antibody, hypothetical protein antibody, Histone deacetylase 4 antibody, GF18710 gene product from transcript GF18710-RC antibody, PTRG_03699 antibody, PGTG_15512 antibody, HDAC4 antibody, Dana\GF18710 antibody, Hdac4 antibody, hdac4 antibody

Background: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Molecular Weight: 140 kDa

NCBI Accession: NP_006028

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process