HSP90AB1 antibody (pSer254)

Catalog No. ABIN362221

This Rabbit Polyclonal antibody specifically detects HSP90AB1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HSP90AB1 antibody (pSer254) (ABIN362221)

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90AB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSP90AB1 Antibody

Binding Specificity: pSer254

Specificity: The antibody detects endogenous level of HSP90B only when phosphorylated at serine 254.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer254 (V-G-S (p) -D-E) derived from Human HSP90B. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for HSP90AB1

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Alternative Name: HSP90B

Background: Molecular chaperone. Has ATPase activity.

Molecular Weight: 92 kDa

NCBI Accession: NP_031381

UniProt: P08238

Pathways: Regulation of Cell Size