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Antibodies
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anti-Insulin Receptor Substrate 1 (IRS1) (pSer639) antibody
Insulin Receptor Substrate 1 (IRS1) (pSer639) antibody
| Antigen |
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| Synonyms |
HIRS-1, G972R, IRS-1, IRS1IRM, irs1, MGC75645, IRS1 |
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Epitope
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pSer307 (19), pSer312 (16), pSer639 (13), pSer636 (11), pTyr896 (4), pSer612 (3), pSer616 (2), pTyr1179 (2), pTyr612 (2), pS307 (1), pTyr1225 (1), pTyr941 (1), pY1179 (1), pY896 (1)
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (100), Immunofluorescence (IF) (65), Immunohistochemistry (IHC) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Flow Cytometry (FACS) (28), ELISA (25), Enzyme Immunoassay (EIA) (14), Immunoprecipitation (IP) (12), Dot Blot (Dot) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN362279 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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IRS-1 (Phospho-Ser639)
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Swiss-Prot
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P35568
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 639 (P-K-S P -V-S).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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IRS-1 (Phospho-Ser639) (Human) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 639.
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50-1:100
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Insulin Receptor Substrate 1 (IRS1)", type "Antibodies"
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Hosts
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Rabbit (165), Mouse (4)
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Reactivities
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Human (150), Mouse (Murine) (107), Rat (Rattus) (95), Cow (Bovine) (5), Chicken (3), Dog (Canine) (3), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (100), Immunofluorescence (IF) (65), Immunohistochemistry (IHC) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Flow Cytometry (FACS) (28), ELISA (25), Enzyme Immunoassay (EIA) (14), Immunoprecipitation (IP) (12), Dot Blot (Dot) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer307 (19), pSer312 (16), pSer639 (13), pSer636 (11), pTyr896 (4), pSer612 (3), pSer616 (2), pTyr1179 (2), pTyr612 (2), pS307 (1), pTyr1225 (1), pTyr941 (1), pY1179 (1), pY896 (1)
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