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Antibodies
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anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pTyr204) antibody
Mitogen-Activated Protein Kinase 3 (MAPK3) (pTyr204) antibody
| Antigen |
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| Synonyms |
ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940 |
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Epitope
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N-Term (7), pThr202 (7), pTyr204 (7), pThr202/pTyr204 (5), C-Term (4), pThr202,pTyr204 (2), pThr202/Tyr204 (2), D192 (1), T202 (1), Y204 (1), pT-E-pY Motif (1), pT202 (1), pY204 (1)
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (139), Rat (Rattus) (64), Mouse (Murine) (62), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Dog (Canine) (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
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Application
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 Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Western Blotting (WB) (132), ELISA (34), Immunofluorescence (IF) (33), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (23), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
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| Catalog no. |
ABIN362301 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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p44/42 MAP Kinase (Phospho-Tyr204)
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Swiss-Prot
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P27361
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from humanp44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-Y P -V-A).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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P44/42 MAP Kinase (Phospho-Tyr204) (Human) antibody detects endogenous levels of p44/42 MAPKinase only when phosphorylated at tyrosine 204
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Application Notes
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WB: 1:500~1:1000 IF:1:100~1:200 IHC:1:50~1:100
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Mitogen-Activated Protein Kinase 3 (MAPK3)", type "Antibodies"
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Hosts
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Rabbit (113), Mouse (30), Goat (6), Chicken (1), Sheep (1)
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Reactivities
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Human (139), Rat (Rattus) (64), Mouse (Murine) (62), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Dog (Canine) (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
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Applications
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Western Blotting (WB) (132), ELISA (34), Immunofluorescence (IF) (33), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (23), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
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Conjugates
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Biotin (1), HRP (1)
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Epitopes
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N-Term (7), pThr202 (7), pTyr204 (7), pThr202/pTyr204 (5), C-Term (4), pThr202,pTyr204 (2), pThr202/Tyr204 (2), D192 (1), T202 (1), Y204 (1), pT-E-pY Motif (1), pT202 (1), pY204 (1)
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