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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pTyr315/316/312) antibody
| Antigen | V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) |
| Synonyms |
AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, ...
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| Epitope |
 Alternatives pTyr315/316/312 |
| Clonality | Polyclonal |
| Host |
Alternatives Rabbit |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives Immunohistochemistry (IHC), Immunofluorescence (IF)
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| Catalog no. | ABIN362434 |
| Quantity | 100µg (100µg/100µl) (Variants) |
| Price | 360.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business days |
Additional Information
| Alternative name | AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) |
| Swiss-Prot | P31749 P31751 Q9Y243 |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from humanAKT1/AKT2/AKT3 around the phosphorylation site of tyrosine 315/316/312 (P-E-Y P -L-A). |
| Isotype | IgG (Matching secondary antibodies) |
| Specificity | AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) (Human) antibody detects endogenous levels of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312. |
| Synonyms | AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006 |
Application Details
| Application Notes | IHC: 1:50~100 IF:1:100~1:200 |
| Concentration | 100µg/100µl |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
| Buffer | Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage | Store at -20°C. Avoid repeat freeze-thaw cycles. |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
| Hosts | Rabbit (162), Mouse (60), Sheep (4), Chicken (1) |
| Reactivities | Human (214), Mouse (Murine) (121), Rat (Rattus) (112), Chicken (7), Dog (Canine) (4), Chimpanzee (2), Cow (Bovine) (2), Frog (1), Rabbit (1), Sheep (Ovine) (1) |
| Applications | Western Blotting (WB) (183), Immunohistochemistry (IHC) (63), Enzyme Immunoassay (EIA) (60), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (59), ELISA (54), Immunofluorescence (IF) (44), Immunoprecipitation (IP) (16), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), Dot Blot (Dot) (9), Flow Cytometry (FACS) (4), Immunocytochemistry (ICC) (2), ELISA (Detection) (1) |
| Conjugates | Biotin (2), APC (1) |
| Epitopes | pSer473 (21), pThr308 (14), pThr450 (5), S129 (3), pTyr315/316/312 (3), C-Term (2), N-Term (2), S246 (2), pS124 (2), pS473 (2), pThr308/309 (2), pY326 (2), N-Term G37 (1), S124 (1), S473 (1), pT308 (1) |
