Cofilin1/2 (CFL1/2) (pTyr88) antibody

Details for Product No. ABIN362441
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Antigen
Synonyms CFL
Epitope
pTyr88
(5), (2)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(9), (9), (5)
Host
Rabbit
(9)
Clonality
Polyclonal
Application
Immunohistochemistry (IHC)
(7), (4), (2), (2)
Pubmed 3 references available
Catalog no. ABIN362441
Quantity 50 µL
Price
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  • +1 404 474 4654
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Immunogen Peptide sequence around phosphorylation site of pTyr88 (A-T-Y (p) -E-T) derived from Human coflin1, cofilin2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of cofilin1/cofilin2 only when phosphorylated at tyrosine 88.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name cofilin1/cofilin2
Background Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Molecular Weight 19 kDa
UniProt Q9Y281
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Arber, Barbayannis, Hanser et al.: "Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase." in: Nature, Vol. 393, Issue 6687, pp. 805-9, 1998 (PubMed).

Yang, Higuchi, Ohashi et al.: "Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization." in: Nature, Vol. 393, Issue 6687, pp. 809-12, 1998 (PubMed).

Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

Hosts (9)
Reactivities (9), (9), (5)
Applications (7), (4), (2), (2)
Epitopes (5), (2)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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