Eukaryotic Translation Initiation Factor 2A (EIF2A) (pSer49) antibody

Details for Product No. ABIN362446
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Antigen
Synonyms
35kDa, Eif2a, eIF2alpha, 0910001O23Rik, 2410026C18Rik, EIF2S1, EIF2A, ATEIF2-A2, EIF2-A2, eukaryotic translation initiation factor 2 alpha subunit, DDBDRAFT_0185936, DDBDRAFT_0234177, DDB_0185936, DDB ... show more
35kDa, Eif2a, eIF2alpha, 0910001O23Rik, 2410026C18Rik, EIF2S1, EIF2A, ATEIF2-A2, EIF2-A2, eukaryotic translation initiation factor 2 alpha subunit, DDBDRAFT_0185936, DDBDRAFT_0234177, DDB_0185936, DDB_0234177, eif2a show less
Epitope
pSer49
(24), (3), (2), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(53), (52), (52), (12), (12)
Host
Rabbit
(53)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Immunofluorescence (IF)
(28), (21), (20), (13), (6), (5)
Pubmed 4 references available
Catalog no. ABIN362446
Quantity 100 µL
Price
286.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide sequence around phosphorylation site of pSer49 (L-L-S (p) -E-L) derived from Human eIF2a. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of eIF2a only when phosphorylated at Serine 49.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name eIF2alpha
Background Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Molecular Weight 38 kDa
NCBI Accession NP_004085
UniProt P05198
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications de Haro, Méndez, Santoyo: "The eIF-2alpha kinases and the control of protein synthesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 10, Issue 12, pp. 1378-87, 1996 (PubMed).

Tabor: "Prions of mammals and fungi minireview series." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 1, 1999 (PubMed).

Korpelainen, Kärkkäinen, Gunji et al.: "Endothelial receptor tyrosine kinases activate the STAT signaling pathway: mutant Tie-2 causing venous malformations signals a distinct STAT activation response." in: Oncogene, Vol. 18, Issue 1, pp. 1-8, 1999 (PubMed).

Kimball: "Eukaryotic initiation factor eIF2." in: The international journal of biochemistry & cell biology, Vol. 31, Issue 1, pp. 25-9, 1999 (PubMed).

Hosts (53)
Reactivities (53), (52), (52), (12), (12)
Applications (28), (21), (20), (13), (6), (5)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (24), (3), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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