Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibody

Details for Product No. ABIN362462
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Antigen
Synonyms marcks, MGC89127, MARCKS, Macs, PKCSL, KINC, macs, wu:fb55c06, wu:fb92d04, wu:fc19b04, wu:fc44a10, zgc:110235, 80K-L, MACS, PRKCSL
Epitope
pSer170
(26), (26), (25), (24), (22), (15), (9), (7), (6), (5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(194), (138), (128), (54), (40), (36), (25), (3), (3), (1), (1), (1)
Host
Rabbit
(194), (17), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(156), (59), (52), (40), (27), (25), (20), (4), (2), (1), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN362462
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Immunogen Peptide sequence around phosphorylation site of pSer170 (G-F-S (p) -F-K) derived from Human MARCKS. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of MARCKS only when phosphorylated at threonine 170.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MARCKS
Background MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Molecular Weight 80 kDa
NCBI Accession NP_002347
UniProt P29966
Research Area Cardiovascular
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Hartwig, Thelen, Rosen et al.: "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin." in: Nature, Vol. 356, Issue 6370, pp. 618-22, 1992 (PubMed).

Thelen, Rosen, Nairn et al.: "Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane." in: Nature, Vol. 351, Issue 6324, pp. 320-2, 1991 (PubMed).

Graff, Young, Johnson et al.: "Phosphorylation-regulated calmodulin binding to a prominent cellular substrate for protein kinase C." in: The Journal of biological chemistry, Vol. 264, Issue 36, pp. 21818-23, 1990 (PubMed).

Ramsden: "MARCKS: a case of molecular exaptation?" in: The international journal of biochemistry & cell biology, Vol. 32, Issue 5, pp. 475-9, 2001 (PubMed).

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