PRKDC antibody (AA 2607-2611)

Catalog No. ABIN362807

The Rabbit Polyclonal anti-PRKDC antibody is suitable to detect PRKDC in samples from Human. It has been validated for WB.

Quick Overview for PRKDC antibody (AA 2607-2611) (ABIN362807)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRKDC Antibody

Binding Specificity: AA 2607-2611

Specificity: The antibody detects endogenous level of total DNA PKcs protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 2607-2611 (V-E-T-Q-A) derived from Human DNA-PK. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: DNA PKcs

Background: The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity, PRKDC must be bound to DNA to express its catalytic properties

Molecular Weight: 450 kDa

NCBI Accession: NP_001075

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response