HSP27 antibody (AA 76-80)

Catalog No. ABIN362913

Cited in 1 publication. The Rabbit Polyclonal anti-HSP27 antibody (ABIN362913) specifically detects HSP27 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for HSP27 antibody (AA 76-80) (ABIN362913)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HSP27 Antibody

Binding Specificity: AA 76-80

Specificity: The antibody detects endogenous level of total HSP27 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Specificity/Sensitivity HSP27 (Ab-78) antibody detects endogenous levels of total HSP27 protein.

Immunogen: Peptide sequence around AA 76-80 (A-L-S-R-Q) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-HSP27 antibody (ABIN362913)

Ma, Teng, Hua, Hou, Luo, Jiang: "Upregulation of heat shock protein 27 confers resistance to actinomycin D-induced apoptosis in cancer cells." in: The FEBS journal, Vol. 280, Issue 18, pp. 4612-24, (2013) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Background: Involved in stress resistance and actin organization.

Molecular Weight: 27 kDa

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling