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Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody

Antigen

Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)

Synonyms MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives Western Blotting (WB), Immunofluorescence (IF)
1 reference available
Catalog no. ABIN362995
Quantity 50µg  (50µg/50µl)  (Variants)
Price 250.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name MARCKS (Ab-158)
Swiss-Prot P29966
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived fromhuman MARCKS around the phosphorylation site of serine 158 (R-F-S P -F-K).
Isotype IgG  (Matching secondary antibodies)
Specificity MARCKS (Ab-158) (Human) antibody detects endogenous levels of total MARCKS protein.

Application Details

Application Notes WB: 1:500~1:1000 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Publications

Publications Litherland, Elias, Hui et al.: "Protein kinase C isoforms {zeta} and {iota} mediate collagenase expression and cartilage destruction via STAT- and ERK-dependent c-fos induction." in: The Journal of biological chemistry, 2010 (PubMed).

Alternatives

Alternatives for antigen "Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)", type "Antibodies"
Hosts Rabbit (96), Mouse (7), Rat (2)
Reactivities Human (87), Rat (Rattus) (65), Mouse (Murine) (59), Cow (Bovine) (19), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (17), Chicken (2), Guinea Pig (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
Applications Western Blotting (WB) (72), Immunofluorescence (IF) (55), Flow Cytometry (FACS) (20), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (10), Immunocytochemistry (ICC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer158 (7), pSer162 (7), pSer170 (6), Ab-162 (4), C-Term (2), N-Term (2), pSer152, pSer156 (1), pSer152/156 (1), pSer163 (1), phospho (1)