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Antibodies
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anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody
Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody
| Antigen |
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| Synonyms |
MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (87), Rat (Rattus) (65), Mouse (Murine) (59), Cow (Bovine) (19), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (17), Chicken (2), Guinea Pig (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Application
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Western Blotting (WB) (72), Immunofluorescence (IF) (55), Flow Cytometry (FACS) (20), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (10), Immunocytochemistry (ICC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN362995 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
250.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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MARCKS (Ab-158)
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Swiss-Prot
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P29966
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Immunogen
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The antiserum was produced against synthesized non-phosphopeptide derived fromhuman MARCKS around the phosphorylation site of serine 158 (R-F-S P -F-K).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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MARCKS (Ab-158) (Human) antibody detects endogenous levels of total MARCKS protein.
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Application Notes
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WB: 1:500~1:1000 IF:1:100~1:200
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Publications
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Litherland, Elias, Hui et al.: "Protein kinase C isoforms {zeta} and {iota} mediate collagenase expression and cartilage destruction via STAT- and ERK-dependent c-fos induction." in: The Journal of biological chemistry, 2010 (PubMed).
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Alternatives for antigen "Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)", type "Antibodies"
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Hosts
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Rabbit (96), Mouse (7), Rat (2)
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Reactivities
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Human (87), Rat (Rattus) (65), Mouse (Murine) (59), Cow (Bovine) (19), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (17), Chicken (2), Guinea Pig (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Western Blotting (WB) (72), Immunofluorescence (IF) (55), Flow Cytometry (FACS) (20), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (10), Immunocytochemistry (ICC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer158 (7), pSer162 (7), pSer170 (6), Ab-162 (4), C-Term (2), N-Term (2), pSer152, pSer156 (1), pSer152/156 (1), pSer163 (1), phospho (1)
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