PI3K p85a/g/b antibody (AA 465-469)

Catalog No. ABIN363075

Cited in 2+ publications. The Rabbit Polyclonal anti-PI3K p85a/g/b antibody (ABIN363075) specifically detects PI3K p85a/g/b in IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PI3K p85a/g/b antibody (AA 465-469) (ABIN363075)

Target: PI3K p85a/g/b (Phosphoinositide 3 Kinase, p85 alpha/gamma/beta (PI3K p85a/g/b))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3K p85a/g/b antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-PI3K p85a/g/b Antibody

Binding Specificity: AA 465-469

Specificity: The antibody detects endogenous level of total PI3K P85 a/g/b protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 465-469, 197-201, 462-466 (L-Y-E-E-Y) derived from Human PI3K P85. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-PI3K p85a/g/b antibody (ABIN363075)

Li, Lu, Chen, Bu, Huang: "Loss of fatty acid synthase inhibits the "HER2-PI3K/Akt axis" activity and malignant phenotype of Caco-2 cells." in: Lipids in health and disease, Vol. 12, pp. 83, (2013) (PubMed).

Li, Bu, Wu, Wu, Huang: "The "HER2-PI3K/Akt-FASN Axis" regulated malignant phenotype of colorectal cancer cells." in: Lipids, Vol. 47, Issue 4, pp. 403-11, (2012) (PubMed).

Target Details for PI3K p85a/g/b

Target: PI3K p85a/g/b (Phosphoinositide 3 Kinase, p85 alpha/gamma/beta (PI3K p85a/g/b))

Alternative Name: PI3K P85 alpha/gamma/beta

Background: PI3K binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Molecular Weight: 85 kDa

UniProt: O00459