GRP78 antibody (Internal Region)

Catalog No. ABIN363671

The Rabbit Polyclonal anti-GRP78 antibody is suitable to detect GRP78 in samples from Human, Rat, Mouse, Drosophila melanogaster, Sheep and Chicken. It has been validated for WB, ICC, IF and FACS.

Quick Overview for GRP78 antibody (Internal Region) (ABIN363671)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human, Rat, Mouse, Drosophila melanogaster, Sheep, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GRP78 Antibody

Binding Specificity: AA 250-300, Internal Region

Specificity: Reacts with GRP-78 protein from human, mouse, rat, Drosophila, sheep and chicken

Cross-Reactivity (Details): Predicted to cross reacts with proteins from Bovine, C elegans, Primate, Zebrafish due to 100 % sequence homology.

Purification: Antigen affinity purified

Immunogen: Synthetic peptide derived from internal domain of the human Grp78 protein. Within residues 250-300.

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(0.5 μg/mL) FC(1:50) ICC/ IF(1:50)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.67 mg/mL

Buffer: PBS, glycerol 30 % , Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Hu, Mu, Rt, Ch, Dr, Sh, Bv, Ce, Pm, Ze ovine (100 % ), Zebrafish (100 %), Primate (100 %), C. elegans (100 %).

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: 78 kDa Glucose-Regulated Protein (GRP-78)

Background: Categorized as stress response proteins, BiPs play an important role in folding and assembly of nascent proteins and in scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5- nontranslated region of BiP mRNA. BiP IRES activity increases when cells are heat-stressed. GRP78 is also important in maintenance of cell homeostasis and the prevention of apoptosis. Levels of GRP78 protein are reduced in brains of Alzheimer's disease patients.

Gene ID: 3309

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling