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Immunoglobulin E (IgE) (Fc specific) antibody
| Antigen | Immunoglobulin E (IgE) |
| Epitope |
 Alternatives Fc specific |
| Clonality | Monoclonal (BSW17-NBS01) |
| Host |
Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
|
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4 references available |
| Catalog no. | ABIN363703 |
| Quantity | 100 ug (1mg/ml) (Variants) |
| Price | 273.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 3 to 5 Business Days |
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Additional Information
| Alternative name | Immunoglobulin E (IgE) (Fc) |
| Gene ID | IGHE, chromosome 14q32.33 |
| Immunogen | purified human myeloma IgE |
| Format | Affinity-purified, 0.1% sodium azide |
| Isotype | IgG1 kappa |
| Clone | BSW17-NBS01 |
| Description | The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling. |
| Specificity | Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes |
Application Details
| Application Notes | When used for functional cellular assays (mast cell / basophil activation) at dilutions < 1:100, the Na-azide free formulation is recommended. Quality assurance: Reactivity > 99%, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11) |
| Concentration | 1mg/ml |
| Purity | > 95%, tested by analytical Gel Permeation Chromatography |
| Purification | Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B |
| Buffer | PBS, pH=7.2, 0.1% NaN3 |
| Storage | Store undiluted at 4°C. For long term storage, aliquot and store at -20°C |
| Research Area | Allergy |
| Restrictions | For Research Use only |
Images
| product purity: gel permeation chromatography (Superose 12/HR) Target binding kinetics (Biacore |
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Publications
| Publications |
Knutti-Mueller, Stadler, Magnusson et al.: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).
Czech, Stadler, Schuopf et al.: "IgE autoantibodies in atopic dermatitis--occurrence of different antibodies against the CH3 and the CH4 epitopes of IgE." in: Allergy, Vol. 50, Issue 3, pp. 243-8, 1995 (PubMed). Rudolf, Furukawa, Miescher et al.: "Effect of anti-IgE antibodies on Fc epsilonRI-bound IgE." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5646-52, 1997 (PubMed). Rudolf, Zuercher, Nechansky et al.: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed). |
Alternatives
Alternatives for antigen "Immunoglobulin E (IgE)", type "Antibodies"
| Hosts | Mouse (5), Rat (3) |
| Reactivities | Human (5), Mouse (Murine) (3) |
| Applications | Enzyme Immunoassay (EIA) (7), Immunofluorescence (IF) (3), Dot Blot (Dot) (2), ELISA (Capture) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Western Blotting (WB) (2), ELISA (Detection) (1), Flow Cytometry (FACS) (1), Functional Studies (Func) (1) |
| Epitopes | Epsilon Chain Specific (3), Heavy Chains (3), C epsilon 2 specific (1), C epsilon 3 specific (1) |
