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Details for Product No. ABIN363706

Immunoglobulin E (IgE) (Fc specific) antibody

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Antigen
Epitope
Fc specific
Reactivity
Human
Host
Mouse
Clonality (Clone) Monoclonal ()
Application
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
Pubmed 4 references available
Catalog no. ABIN363706
Quantity 100 µg
Price
304.92 $   Plus shipping costs $45.00
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Availability Will be delivered in 4 to 5 Business Days
Immunogen purified human myeloma IgE
Clone BSW17-NBS01
Isotype IgG1 kappa
Specificity Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
Purification Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
Purity > 95%, tested by analytical Gel Permeation Chromatography
Sterility Sterile filtered
Alternative Name Immunoglobulin E (IgE) (Fc)
Background The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
Gene ID IGHE, chromosome 14q32.33
Research Area Allergy
Application Notes For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended. Quality assurance: Reactivity > 99%, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
Restrictions For Research Use only
Concentration 1mg/ml
Buffer PBS, pH=7.2, sterile filtered, contains no NaN3
Preservative Azide free
Storage 4 °C
Knutti-Mueller, Stadler, Magnusson et al.: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).

Czech, Stadler, Schuopf et al.: "IgE autoantibodies in atopic dermatitis--occurrence of different antibodies against the CH3 and the CH4 epitopes of IgE." in: Allergy, Vol. 50, Issue 3, pp. 243-8, 1995 (PubMed).

Rudolf, Furukawa, Miescher et al.: "Effect of anti-IgE antibodies on Fc epsilonRI-bound IgE." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5646-52, 1997 (PubMed).

Rudolf, Zuercher, Nechansky et al.: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

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