THO Complex 4 antibody

Catalog No. ABIN364243

This Mouse Monoclonal antibody specifically detects THO Complex 4 in WB, ELISA, IHC, IF, IP and IHC (p). It exhibits reactivity toward Human and Xenopus laevis.

Quick Overview for THO Complex 4 antibody (ABIN364243)

Target: THO Complex 4 (THOC4)

Reactivity: Human, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This THO Complex 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 11G5

Product Details anti-THO Complex 4 Antibody

Specificity: Human ALY. Detects a band of approximately 32 kD. Cross reacts with xenopus

Purification: Protein A purified

Immunogen: Recombinant Human ALY/REF protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-P (2.5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for THO Complex 4

Target: THO Complex 4 (THOC4)

Alternative Name: ALY / THOC4

Background: Name/Gene ID: ALYREF

Synonyms: ALYREF, ALY, BEF, BZIP enhancing factor, BZIP-enhancing factor BEF, Ally of AML-1 and LEF-1, Aly/REF export factor, THO complex 4, THO complex subunit 4, REF, THOC4, Tho4

Gene ID: 10189

UniProt: Q86V81