Keratin 10 antibody

Catalog No. ABIN364279

This Mouse Monoclonal antibody specifically detects Keratin 10 in IHC, WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Keratin 10 antibody (ABIN364279)

Target: Keratin 10 (KRT10)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AE20

Product Details anti-Keratin 10 Antibody

Specificity: Human keratin K10-1 (56.5 Kda)

Purification: Protein G purified

Immunogen: Human epidermal keratins.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: KRT10 / CK10 / Cytokeratin 10

Background: Name/Gene ID: KRT10

Synonyms: KRT10, BIE, BCIE, CK10, Cytokeratin 10, Cytokeratin-10, EHK, Keratin-10, KPP, Keratin 10, CK-10, K10

Gene ID: 3858

UniProt: P13645