HSP90 antibody (AA 1-732)

Catalog No. ABIN364328

The Mouse Monoclonal anti-HSP90 antibody is suitable to detect HSP90 in samples from Human. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for HSP90 antibody (AA 1-732) (ABIN364328)

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP90 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4F10

Product Details anti-HSP90 Antibody

Binding Specificity: AA 1-732

Purification: Protein G purified

Immunogen: Recombinant human Hsp90 (1-732aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Alternative Name: Heat Shock Protein 90

Background: Name/Gene ID: HSP90

Synonyms: HSP90

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals