PAFAH1B1 antibody (AA 397-410)

Catalog No. ABIN364420

This Goat Polyclonal antibody specifically detects PAFAH1B1 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Horse, Rabbit, Pig, Chicken, Xenopus laevis, Bat, Hamster and Monkey.

Quick Overview for PAFAH1B1 antibody (AA 397-410) (ABIN364420)

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Horse, Rabbit, Pig, Chicken, Xenopus laevis, Bat, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This PAFAH1B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAFAH1B1 Antibody

Binding Specificity: AA 397-410

Specificity: Human PAFAH1B1 / LIS1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Water flea, Tick (100%) Chimpanzee, Drosophila, Ant, Capsaspora (93%) Catfish, Mosquito, Sea anemone, Beetle, Trichoplax (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide TGSVDQTVKVWECR from the C-terminus of human PAFAH1B1 / LIS1 (NP_000421.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Water flea, Tick (100%), Chimpanzee, Drosophila, Ant, Capsaspora (93%), Catfish, Mosquito, Sea anemone, Beetle, Trichoplax (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PAFAH1B1

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Alternative Name: PAFAH1B1 / LIS1

Background: Name/Gene ID: PAFAH1B1

Synonyms: PAFAH1B1, LIS-1, LIS2, Lissencephaly 1 protein, Lissencephaly-1 protein, MDCR, MDS, PAFAHA, PAF-AH alpha, PAFAH, LIS1, PAF-AH 45 kDa subunit, PAFAH alpha

Gene ID: 5048

NCBI Accession: NP_000421

UniProt: P43034

Pathways: M Phase, Regulation of Cell Size