MBP antibody

Catalog No. ABIN364503

The Rabbit Polyclonal anti-MBP antibody (ABIN364503) specifically detects MBP in WB, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for MBP antibody (ABIN364503)

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MBP Antibody

Specificity: Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.

Purification: Ammonium sulfate precipitation

Immunogen: Human myelin basic protein from brain.

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100), WB (1:1500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. Western Blot: 1:1500 in TTBS, overnight at 4uC.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05M Tris HCL,  pH 7.6, 15 mM sodium azide before the addition of glycerol to 40 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein / MBP

Background: Name/Gene ID: MBP

Synonyms: MBP, Myelin A1 protein, Myelin basic protein

Gene ID: 4155

UniProt: P02686