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CD72 Molecule (CD72) antibody (Biotin)

Details for Product No. ABIN370999, Supplier: Log in to see
Antigen
  • CD72c
  • Ly-19
  • Ly-32
  • Ly-m19
  • Lyb-2
  • CD72b
  • LYB2
Reactivity
Mouse (Murine)
99
32
7
Host
Mouse
77
53
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
18
15
11
3
3
3
1
Application
Flow Cytometry (FACS)
55
52
46
19
10
7
3
3
2
2
2
1
Supplier
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Immunogen DBA/2 mouse spleen cells. (Ref. 1)
Clone 10-1-D-2
Isotype IgG2b
Specificity This antibody recognises CD72.1/Lyb-2.1 (Mr. 45 kDa).
Characteristics Synonyms: Lyb-2, Ly-32, Ly32, B-Cell marker, B-cell differentiation antigen CD72, Lymphocyte antigen32
Alternative Name CD72 (CD72 Antibody Abstract)
Background Murine CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells. (Ref.1,2) This clone blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing. (Ref.1) However, 10-1.D.2 can itself trigger a positive signal by binding CD72. (Ref.1-5)Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32
Gene ID 100504743
UniProt P21855
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.1,2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Robinson, Landolfi, Schäfer et al.: "Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 9, pp. 4764-72, 1993 (PubMed).

Subbarao, Mosier: "Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 130, Issue 5, pp. 2033-7, 1983 (PubMed).

Snow, Mond, Subbarao: "Enhancement by monoclonal anti-Lyb-2 antibody of antigen-specific B lymphocyte expansion stimulated by TNP-Ficoll and T lymphocyte-derived factors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 6, pp. 1793-6, 1986 (PubMed).

Luo, Van de Velde, von Hoegen et al.: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, 1992 (PubMed).

Beavil, Edmeades, Gould et al.: "Alpha-helical coiled-coil stalks in the low-affinity receptor for IgE (Fc epsilon RII/CD23) and related C-type lectins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 753-7, 1992 (PubMed).