GRP78 antibody (Biotin)

Catalog No. ABIN371046

Product Details anti-GRP78 Antibody (Biotin)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP78 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody is specific to BIP (Immunoglobulin heavy chain binding protein, Mr 78 kDa). Monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP. (Ref.8)

Characteristics: Synonyms: 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5, BiP, Immunoglobulinheavy chain-binding protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78

Clone: 76-E6

Isotype: IgG1

Application Details

Application Notes: ELISA. (Ref.6)Immunoblotting: < / = 10 μg/10^6 cells. (Ref.6,8)Studies of Immunoglobulin assembly and secretion. Ref.1,2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5 / GRP78 (HSPA5 Products)

Synonyms: GRP78 antibody, BiP antibody, GRP-78 antibody, grp78 antibody, hspa5a antibody, BIP antibody, MIF2 antibody, AL022860 antibody, AU019543 antibody, Bip antibody, D2Wsu141e antibody, D2Wsu17e antibody, Grp78 antibody, Hsce70 antibody, SEZ-7 antibody, Sez7 antibody, baffled antibody, mBiP antibody, cb865 antibody, fb60h09 antibody, fi36d04 antibody, wu:fb60h09 antibody, wu:fi36d04 antibody, zgc:55994 antibody, zgc:77606 antibody, 78 kDa glucose-regulated protein antibody, heat shock protein family A (Hsp70) member 5 antibody, BiP/GRP78 antibody, glucose-regulated protein 78 antibody, putative glucose-regulated protein 78 antibody, Hsp70 family ATPase KAR2 antibody, heat shock protein family A (Hsp70) member 5 S homeolog antibody, heat shock 70 kDa protein 5a antibody, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody, heat shock protein 5 antibody, heat shock protein family A member 5 antibody, CpipJ_CPIJ003550 antibody, HSPA5 antibody, grp78 antibody, LOC100533358 antibody, BiP/grp78 antibody, Tc00.1047053506585.40 antibody, Tb11.02.5450 antibody, Tb11.02.5500 antibody, LMJF_28_1200 antibody, KAR2 antibody, LOC100135840 antibody, hspa5.S antibody, hspa5 antibody, Hspa5 antibody

Background: The immunoglobulin heavy chain binding protein BiP (Binding Protein) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78. (Ref.2) While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as Molecular Chaperones which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding. (Ref.1-5) With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (μ+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP. (Ref.7)Synonyms: 78 kDa glucose-regulated protein, BiP, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein

Gene ID: 14828

UniProt: P20029

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling