Mouse anti-Rat IgG1 Antibody (HRP)

Catalog No. ABIN371212

Quick Overview for Mouse anti-Rat IgG1 Antibody (HRP) (ABIN371212)

Target: IgG1

Clonality: Monoclonal

Conjugate: HRP

Application: Enzyme Immunoassay (EIA)

Clone: G1 7E7

Isotype: IgG1

Product Details

Reactivity: Rat

Host: Mouse

Application Details

Application Notes: ELISA: 1/2,000-1/5,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 % Glycerol/50 % PBS, pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -28 °C for longer.

Target Details

Target: IgG1

Target Type: Antibody

Background: Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10e11 variants. Immunoglobulins, or antibodies, are a complex, heterogeneous mixture of proteins that exhibit two fundamental types of structural variation. Subtle structural differences in their antigen combining sites, or variable regions, account for their unique antigen binding specificities.Synonyms: Rat Immunoglobulin G1