Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) antibody (HRP)

Antigen: Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1)

Synonyms: CLG, CLGN, Dm1-MMP, l(2)k04809, mmp1, DmelCG4859, CG4859, MMP1, Mmp1a, Mmp1

Clonality: Monoclonal (SB12e)

Host: Mouse

Reactivity: Human

Conjugate: HRP

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN371652

Additional Information

Characteristics: Synonyms: Interstitial collagenase, Matrix metalloproteinase-1, Fibroblast collagenase, MMP1, CLG

Alternative name: MMP-1

Swiss-Prot: P03956

Immunogen: Recombinant MMP-1.

Cross-Reactivity: Human

Isotype: IgG2b

Clone: SB12e

Description: The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted andmembrane bound zincendopeptidases. Collectively, these enzymes can degrade all thecomponents of the extracellular matrix, including fibrillar and non fibrillar collagens,fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, apropeptide, and a catalytic domain containing the highly conserved zinc binding sitecharacterizes the structure of the MMPs. In addition, fibronectin like repeats, a hingeregion, and a C terminal hemopexin like domain allow categorization of MMPs into thecollagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs aresynthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrixbreakdown. MMPs are considered to play an important role in wound healing, apoptosis,bone elongation, embryo development, uterine involution, angiogenesis and tissueremodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas,lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and incancer cell invasion and metastasis. MMP1, also known as interstitial collagenase, is the only enzyme that is able to initiate thebreakdown of the interstitial collagens, types I, II, and III

Specificity: This antibody is specific for Human MMP-1. Does not cross react to human MMP-2, MMP-3 or MMP-9. Species: Human. Others not tested.

Application Details

Application Notes: ELISA: 1/1,000-1/4,000. Western Blot. Immunohistochemistry (Frozen/Paraffin): 1/1,000-1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: 50% Glycerol/50% PBS, pH 7.4 without preservatives.Label: Horseradish Peroxidase (HRP)

Storage: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Extracellular Matrix

Restrictions: For Research Use only