Fibrillarin antibody

Catalog No. ABIN371824

Product Details anti-Fibrillarin Antibody

Target: Fibrillarin (FBL)

Reactivity: Human, Rat, Drosophila melanogaster, C. elegans, Plant, Schizosaccharomyces pombe

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific for the ~34 kDa Fibrillarin /Nop1p protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Cross-Reactivity (Details): Species reactivity (expected):Mammals.
Species reactivity (tested):Human, Rat, Plant, Drosophila, C. elegans and S. pombe.

Characteristics: Synonyms: FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker, rRNA2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1

Purification: Ig Fraction

Immunogen: Yeast nuclear preparations.

Clone: 38F3

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: FBL (FBL Products)

Background: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3).Synonyms: FIB1, FLRN, Fibrillarin, LOT3, NOP1, Nucleolar Marker, Nucleolar protein 1, RNU3IP1, U3 small nucleolar RNA-associated protein NOP1, rRNA 2'-O-methyltransferase fibrillarin

Gene ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process