Fibrillarin antibody (N-Term)

Catalog No. ABIN2778775

There are 2+ publications for this product available. The Rabbit Polyclonal anti-Fibrillarin antibody is suitable to detect Fibrillarin in samples from Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse and Rabbit. It has been validated for WB and IHC.

Quick Overview for Fibrillarin antibody (N-Term) (ABIN2778775)

Target: Fibrillarin (FBL)

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Fibrillarin Antibody

Binding Specificity: N-Term

Purpose: FBL Antibody - N-terminal region

Sequence: GGGFHSGGNR GRGRGGKRGN QSGKNVMVEP HRHEGVFICR GKEDALVTKN

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against FBL. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FBL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40366-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Fibrillarin antibody (ABIN2778775)

Ernoult-Lange, Wilczynska, Harper, Aigueperse, Dautry, Kress, Weil: "Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies." in: Molecular biology of the cell, Vol. 20, Issue 1, pp. 176-87, (2009) (PubMed).

Armistead, Khatkar, Meyer, Mark, Patel, Coghlan, Lamont, Liu, Wiechert, Cattini, Koetter, Wrogemann, Greenberg, Entian, Zelinski, Triggs-Raine: "Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome." in: American journal of human genetics, Vol. 84, Issue 6, pp. 728-39, (2009) (PubMed).

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: FBL

Background:

Background Information: FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. FBL contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.

Gene Name: Fibrillarin

Alternative Symbols: FIB, FLRN, Nop1, RNU3IP1

Protein Name: rRNA 2'-O-methyltransferase fibrillarin

Molecular Weight: 35kDa

Gene ID: 2091

NCBI Accession: NP_001427

UniProt: P22087

Pathways: Ribonucleoside Biosynthetic Process