THRA antibody (N-Term)

Catalog No. ABIN371843

The Mouse Monoclonal anti-THRA antibody is suitable to detect THRA in samples from Human, Mouse, Rat and Dog. It has been validated for WB.

Quick Overview for THRA antibody (N-Term) (ABIN371843)

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This THRA antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2103

Product Details anti-THRA Antibody

Binding Specificity: N-Term

Specificity: AM08268PU-N is specific for the ~50k THR alpha-1 and the ~58k THR alpha-2 proteins.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Mouse and Rat.
Species reactivity (tested):Human.

Characteristics: Synonyms: Thyroid hormone receptor alpha, THRA1, THRA2, C-erbA-alpha, c-erbA-1, EAR-7, EAR7,NR1A1, Nuclear receptor subfamily 1 group A member 1

Purification: Prepared from Mouse Ascites by Ammonium Sulfate Precipitation followed by Affinity Chromatography on Protein G.

Immunogen: Peptide corresponding to amino acid residues from the N-terminal region of human thyroidhormone receptor, alpha1/alpha2-isotype.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: THRA / ERBA1

Background: Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005, Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TR-alpha1, TR-alpha2, TR-beta1 and TR-beta2. TR-alpha1, TR-beta1 and TR-beta2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005).Synonyms: C-erbA-alpha, EAR-7, EAR7, NR1A1, Nuclear receptor subfamily 1 group A member 1, THRA1, THRA2, Thyroid hormone receptor alpha, c-erbA-1

Gene ID: 7067

UniProt: P10827

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development