TUBB3 antibody

Catalog No. ABIN371847

The Mouse Monoclonal anti-TUBB3 antibody (Clone AA10) (ABIN371847) specifically detects TUBB3 in WB and IHC (p). The antibody is reactive with Rat samples.

Quick Overview for TUBB3 antibody (ABIN371847)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AA10

Product Details anti-TUBB3 Antibody

Specificity: AM08272PU-N is specific for the ~55 kDa beta III Tubulin protein. Note: This clone is similar to the Monoclonal antibody Tuj1.

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Characteristics: Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide corresponding to beta III tubulin.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/10,000. Immunohistochemistry: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: TUBB3 / TUBB4

Background: Tubulin is the major constituent of microtubules, existing as a heterodimer of the alpha and beta subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)Synonyms: Tubulin beta-3 chain, Tubulin beta-4, Tubulin beta-III

Gene ID: 246118

UniProt: Q4QRB4

Pathways: Microtubule Dynamics, M Phase