MARCH4 antibody (AA 300-350)

Catalog No. ABIN372267

The Rabbit Polyclonal anti-MARCH4 antibody (ABIN372267) specifically detects MARCH4 in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat, Dog, Chicken, Horse, Opossum, Zebrafish (Danio rerio) and Chimpanzee samples.

Quick Overview for MARCH4 antibody (AA 300-350) (ABIN372267)

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Horse, Opossum, Zebrafish (Danio rerio), Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MARCH4 Antibody

Binding Specificity: AA 300-350

Specificity: This antibody recognizes Membrane-Associated Ring Finger 4 (MARCH4).

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Chicken, Dog (Canine), Horse, Mouse, Opossum, Rat and ZebrafishSpecies reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to a portion of the amino acids 300-350 of human RNF174.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western Blot: 0.5-2.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % Sodium Azide as preservative and 0.05 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MARCH4

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Alternative Name: MARCH4 / RNF174

Background: MARCH4, an E3 ubiquitin protein ligase, may mediate ubiquitination of MHC I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin protein ligases accept ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.Synonyms: E3 ubiquitin-protein ligase MARCH4, KIAA1399, MARCH-IV, Membrane-associated RING finger protein 4, Membrane-associated RING-CH protein IV, RING finger protein 174

Molecular Weight: 46 kDa

Gene ID: 57574

NCBI Accession: NP_065865

UniProt: Q9P2E8