BAFF antibody (C-Term)

Catalog No. ABIN372454

This Rabbit Polyclonal antibody specifically detects BAFF in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for BAFF antibody (C-Term) (ABIN372454)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAFF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BAFF Antibody

Binding Specificity: AA 254-269, C-Term

Specificity: This antibody recognizes CD257/BAFF.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 254-269 (C-terminal) of Human BAFF.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 10 μg/mL. Western blot: 0.5-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.2 mLPBS containing 0.05 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: CD257 / BAFF

Background: Simultaneously four different laboratories identified a new member of the tumor necrosis factor (TNF) family. This has been named as TALL-1, THANK (TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase, BAFF (for B cell activating factor belonging to the TNF family) and Blys (B lymphocyte stimulator). Membrane-bound BAFF is processed and secreted through the action of a protease whose specificity matches that of the furin family of proprotein convertases. Secreted BLyS/BAFF/THANK acts as a potent B cell growth factor. This protein is expressed abundantly in peripheral blood lymphocytes, specifically in monocytes and macrophages. Its regulation is upregulated by interferon gamma. Overexpression of BLyS/BAFF in transgenic mice lead to increased numbers of mature B and effector T cells. These mice also develop autoimmune like symptoms, such as, high levels of rheumatoid factors, anti-DNA autoantibodies, etc. Two receptors for BLyS/BAFF have been identified and termed as BCMA and TACI.Synonyms: B cell-activating factor, B lymphocyte stimulator, BAFF, BLYS, Dendritic cell-derived TNF-like molecule, TALL1, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, Tumor necrosis factor ligand superfamily member 13B, ZTNF4

Gene ID: 10673

NCBI Accession: NP_001139117

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response