YWHAB antibody (C-Term)

Catalog No. ABIN372584

The Rabbit Polyclonal anti-YWHAB antibody has been validated for WB. It is suitable to detect YWHAB in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Xenopus laevis, Chicken and Primate.

Quick Overview for YWHAB antibody (C-Term) (ABIN372584)

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Xenopus laevis, Chicken, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-YWHAB Antibody

Binding Specificity: C-Term

Specificity: Specific for the ~29k 14-3-3 protein. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the C-terminal peptide that was used to generate the antibody.

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, non-Human Primates, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for YWHAB

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Alternative Name: 14-3-3 Protein beta/alpha

Background: 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004, Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.Synonyms: KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, YWHAB

Gene ID: 56011

NCBI Accession: NP_062250

UniProt: P35213

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location