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AQP2 antibody (pSer261)

AQP2 Reactivity: Human, Rat, Mouse, Cow, Dog, Chicken, Non-Human Primate WB, IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN372590
  • Target See all AQP2 Antibodies
    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
    Binding Specificity
    • 22
    • 19
    • 17
    • 17
    • 16
    • 15
    • 8
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer261
    Reactivity
    • 112
    • 94
    • 84
    • 15
    • 15
    • 11
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Cow, Dog, Chicken, Non-Human Primate
    Host
    • 133
    • 2
    Rabbit
    Clonality
    • 133
    • 3
    Polyclonal
    Conjugate
    • 50
    • 10
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AQP2 antibody is un-conjugated
    Application
    • 125
    • 41
    • 41
    • 40
    • 29
    • 21
    • 21
    • 19
    • 19
    • 14
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Specific for ~29k AQP2 protein phosphorylated at Ser261. This antibody Also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
    Cross-Reactivity (Details)
    Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates.
    Species reactivity (tested):Rat.
    Purification
    Affinity Chromatography.
    Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of Rat Aquaporin 2.
    Isotype
    IgG
    Top Product
    Discover our top product AQP2 Primary Antibody
  • Application Notes
    Western Blot: 1/1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody undiluted (in aliquots) at-20 °C.
  • Bogum, Faust, Zühlke, Eichhorst, Moutty, Furkert, Eldahshan, Neuenschwander, von Kries, Wiesner, Trimpert, Deen, Valenti, Rosenthal, Klussmann: "Small-molecule screening identifies modulators of aquaporin-2 trafficking." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 5, pp. 744-58, (2013) (PubMed).

    Hoffert, Fenton, Moeller, Simons, Tchapyjnikov, McDill, Yu, Pisitkun, Chen, Knepper: "Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24617-27, (2008) (PubMed).

  • Target
    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
    Alternative Name
    Aquaporin-2 (AQP2 Products)
    Synonyms
    AQP-CD antibody, WCH-CD antibody, cph antibody, jpk antibody, AQP-2 antibody, aquaporin-2 antibody, aquaporin 2 antibody, aquaporin 2 (collecting duct) antibody, Aqp2 antibody, AQP2 antibody
    Background
    Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007).Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct
    Gene ID
    25386
    NCBI Accession
    NP_037041
    UniProt
    P34080
    Pathways
    Response to Water Deprivation
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