GABRA6 antibody (Cytoplasmic Loop)

Catalog No. ABIN372635

The Rabbit Polyclonal anti-GABRA6 antibody is suitable to detect GABRA6 in samples from Human, Rat, Mouse and Cow. It has been validated for WB and DB.

Quick Overview for GABRA6 antibody (Cytoplasmic Loop) (ABIN372635)

Target: GABRA6 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6))

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA6 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Product Details anti-GABRA6 Antibody

Binding Specificity: Cytoplasmic Loop

Specificity: Specific for the ~57k Alpha-6 subunit of the GABAA Receptor in Western blots of Rat brain membrane fractions. Also recognizes the alpha-6 subunit of GABAA-R in Bovine, Human amnd Mouse brain extracts. Does not recognizes GABAA-R subunits as reactivity is eliminated in alpha-6 knockout brain.

Purification: Affinity Chromatography.

Immunogen: Fusion protein from the cytoplasmic loop of the Alpha-6 subunit of Rat GABAA Receptor.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for GABRA6

Target: GABRA6 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6))

Alternative Name: GABAA Receptor alpha 6

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six Alpha's, four Beta's and four Gamma's, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for Alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a Gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different Alpha-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003).Synonyms: GABRA-6, GABRA6, Gamma-Aminobutyric Acid Receptor subunit Alpha-6

Gene ID: 29708

NCBI Accession: NP_068613

UniProt: P30191

Pathways: Synaptic Membrane