TAO Kinase 2 antibody (pSer181)

Catalog No. ABIN372726

The Rabbit Polyclonal anti-TAO Kinase 2 antibody (ABIN372726) specifically detects TAO Kinase 2 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Xenopus laevis, Finch and Zebrafish (Danio rerio) samples.

Quick Overview for TAO Kinase 2 antibody (pSer181) (ABIN372726)

Target: TAO Kinase 2 (TAOK2)

Reactivity: Human, Mouse, Rat, Dog, Xenopus laevis, Finch, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAO Kinase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TAO Kinase 2 Antibody

Binding Specificity: pSer181

Specificity: Specific for the ~120k TAO2 phosphorylated at Ser181 in Western blots. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Finch, Xenopus and Zebrafish.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser181 of TAO2.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for TAO Kinase 2

Target: TAO Kinase 2 (TAOK2)

Background: In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999, Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.Synonyms: KFC-C, KIAA0881, Kinase from chicken homolog C, MAP3K17, PSK-1, PSK1, Prostate-derived STE20-like kinase 1, Serine/threonine-protein kinase TAO2, Thousand and one amino acid protein 2, UNQ2971/PRO7431

Gene ID: 9344

NCBI Accession: NP_004774

UniProt: Q9UL54

Pathways: Cell-Cell Junction Organization