IL7R antibody (pTyr449)

Catalog No. ABIN372989

The Rabbit Polyclonal anti-IL7R antibody is suitable to detect IL7R in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and EIA.

Quick Overview for IL7R antibody (pTyr449) (ABIN372989)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL7R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-IL7R Antibody

Binding Specificity: pTyr449

Specificity: This antibody recognizes Interleukin 7 Receptor Alpha Chain (IL7R). This phospho-specific polyclonal antibody reacts with Mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat.
Species reactivity (tested):Mouse.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide corresponding to residues near Tyr449 of Mouse IL-7 protein

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 5 μg/mL. Western blot: 1/1000 - 1/10000. ELISA: 1/100000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, pH 7.2 with 0.15 M Sodium Chloride, 0.01 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: CD127 / IL7R

Background: Interleukin 7 Receptor (IL7R) is a receptor for interleukin 7 (IL7). The function of IL7R requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID).Synonyms: IL-7R-alpha, IL7 receptor alpha, Interleukin-7 receptor subunit alpha

Gene ID: 3575

NCBI Accession: NP_002176

UniProt: P16871

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size