ALDH1A1 antibody (C-Term)

Catalog No. ABIN374078

There is 1 publication for this product available. The Goat Polyclonal anti-ALDH1A1 antibody is suitable to detect ALDH1A1 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for ALDH1A1 antibody (C-Term) (ABIN374078)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ALDH1A1 Antibody

Binding Specificity: C-Term

Sequence: C-EVKTVTVKIS QKNS

Specificity: This antibody detects ALDH1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog) and Cow.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein according to NP_000680.2. Genename: ALDH1A1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in human liver lysates(calculated MW of 54.5 kDa according to NP_000680.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ALDH1A1 antibody (ABIN374078)

Amann, Hofmann, Freudenberger, Holland-Cunz, Eichmüller, Bazhin: "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes." in: Journal of cellular biochemistry, Vol. 113, Issue 3, pp. 792-9, (2012) (PubMed).

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1

Background: ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum, this is the most vulnerable site in PD (Chung et al, 2005). Some studies also identify ALDH1A1 as a marker of cancer stem cells. Studies of breast cancer tissues showed that those that expressed a form of ALDH1A1 had the worst outcomes.Synonyms: ALDC, ALDH-E1, ALDH1A1, ALHDII, Aldehyde dehydrogenase cytosolic, Aldehyde dehydrogenase family 1 member A1, PUMB1, RALDH1, Retinal dehydrogenase 1

Gene ID: 216

NCBI Accession: NP_000680

UniProt: P00352

Pathways: Dopaminergic Neurogenesis