CAPZB antibody (C-Term)

Catalog No. ABIN374101

This Goat Polyclonal antibody specifically detects CAPZB in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow and Dog.

Quick Overview for CAPZB antibody (C-Term) (ABIN374101)

Target: CAPZB (Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CAPZB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CAPZB Antibody

Binding Specificity: C-Term

Sequence: NDLVEALKRK QQC

Specificity: This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig, CowSpecies reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence Genename: CAPZB

Application Details

Application Notes: Peptide ELISA: Limit dilution: 1/32000. Western Blot: 0.5-2 μg/mL. Approx 30 kDa band observed in Hela, Jurkat and Placentalysate (predicted MW of 30 kDa according to NP_004921).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide amd 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid Repeated thawing and freezing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CAPZB

Target: CAPZB (Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB))

Alternative Name: CAPZB

Background: CAPZB is a member of the F-actin capping protein family. This gene encodes the beta subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.Synonyms: CAPZ-B, CapZ beta, F-actin-capping protein subunit beta

Gene ID: 832

NCBI Accession: NP_004921

UniProt: P47756

Pathways: Regulation of Actin Filament Polymerization