DOCK1 antibody (C-Term)

Catalog No. ABIN374114

The Goat Polyclonal anti-DOCK1 antibody is suitable to detect DOCK1 in samples from Human, Mouse, Rat, Cow, Dog and Pig. It has been validated for WB.

Quick Overview for DOCK1 antibody (C-Term) (ABIN374114)

Target: DOCK1 (Dedicator of Cytokinesis 1 (DOCK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This DOCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DOCK1 Antibody

Binding Specificity: C-Term

Sequence: C-TTRKQTSVDS GIVQ

Specificity: This antibody detects DOCK1 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_001371.1 Genename: DOCK1

Application Details

Application Notes: Western blot: 3-4 μg/mL. Approx 150-200 kDa band seen in A431 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for DOCK1

Target: DOCK1 (Dedicator of Cytokinesis 1 (DOCK1))

Alternative Name: DOCK1 / DOCK180

Background: The DOCK1 protein is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. It also functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. The GEF activity of the DOCK1 protein may be enhanced by ELMO1.Synonyms: 180 kDa protein downstream of CRK, Dedicator of cytokinesis protein 1

Gene ID: 1793

NCBI Accession: NP_001371

UniProt: Q14185

Pathways: Signaling of Hepatocyte Growth Factor Receptor