Dimethylarginine Dimethylaminohydrolase 1 (DDAH1) (C-Term) antibody

Antigen: Dimethylarginine Dimethylaminohydrolase 1 (DDAH1)

Synonyms: DDAH, FLJ21264, FLJ25539, AI987801, AW050362, 2410006N07Rik, 2510015N06Rik, fc30c11, wu:fc30c11, ddah1, MGC69055, DDAH1, MGC165853

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Cow (Bovine)

Application: Western Blotting (WB)

Catalog no.: ABIN374149

Additional Information

Characteristics: Synonyms: DDAH-1, DDAHI, DDAH, Dimethylargininase-1, N(G), N(G)-dimethylargininedimethylaminohydrolase 1, Dimethylarginine dimethylaminohydrolase 1

Alternative name: DDAH1

Gene ID: 23576

Swiss-Prot: O94760

Immunogen: Peptide from C Terminus of the protein sequence according to NP_036269. 1,NP_001127917. 1Genename: DDAH1AA Sequence: TCCSVLINKKVDS

Cross-Reactivity: Cow (Bovine), Human

Description: DDAH1 belongs to the dimethylarginine dimethylaminohydrolase (DDAH) family. Thisenzyme plays a role in nitric oxide generation by regulating cellular concentrations ofmethylarginines, which in turn inhibit nitric oxide synthase activity. This gene belongs tothe dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzymeplays a role in nitric oxide generation by regulating cellular concentrations ofmethylarginines, which in turn inhibit nitric oxide synthase activity.

Specificity: This antibody detects N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 at C-term. Species: Human. Others not tested. Add. Information: Expected from sequence similarity to react with Cow.

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.1-0.3 µg/ml. Approx 37kDa band observed in human kidney, liver andpancreas lysates (calculated MW of 31.1kDa according to NP_036269.1). The observedmolecular weight corresponds to earlier findings in literature with different antibodies(Pullamsetti et al, FASEB J.2005 Jul,19(9): 1175-7, PMID: 15827267). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin

Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing. Shelf life: one year from despatch.

Restrictions: For Research Use only