DKK3 antibody (C-Term)

Catalog No. ABIN374182

This Goat Polyclonal antibody specifically detects DKK3 in WB. It exhibits reactivity toward Human.

Quick Overview for DKK3 antibody (C-Term) (ABIN374182)

Target: DKK3

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DKK3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DKK3 Antibody

Binding Specificity: C-Term

Sequence: C-AAAALLGGEE I

Specificity: This antibody detects Dickkopf-3 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_037385.2, NP_056965.3, NP_001018067.1 Genename: DKK3

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx.70 kDa band observed in lysates of cell line HEK293(calculated MW of 38.0 kDa according to NP_037385.2, NP_056965.3 andNP_001018067.1). The observed molecular weight corresponds to earlier findings inliterature (Krupnik et al, Gene.1999 Oct 1,238(2): 301-13. , PMID: 10570958 ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for DKK3

Target: DKK3

Alternative Name: Dickkopf-3 (DKK3)

Background: DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC

Gene ID: 27122

NCBI Accession: NP_037385

UniProt: Q9UBP4